Glucose Biosensor Based on Chitosan-Gold and Prussian Blue-Gold Nanoparticles
نویسندگان
چکیده
Amperometric glucose biosensors were constructed by codeposition of glucose oxidase (GOx) with chitosangold (chitosan-AuNP) on gold-Prussian Blue (Au-PB) nanoparticles modified glassy carbon electrodes (GCE). The high stability of the biosensors was achieved using electrochemically controllable in situ Au-PB film preparation following the film covering with GOx layer encrusted with chitosan-AuNP. The biosensors showed 95 % response time up to 10 s, sensitivity up to 9.5 A M -1 cm -2 and linear calibration graph up to 3 mM of glucose.
منابع مشابه
DNA Biosensor for Determination of 5-Fluorouracil based on Gold Electrode Modified with Au and Polyaniline Nanoparticles and FFT Square Wave Voltammetry
In the present study, a new biosensor for 5-Fluorouracil was described using modified goldelectrode and Fast Fourier transform square wave voltammetry (FFT SWV). Calf thymus DNAimmobilization was on a gold electrode decorated with polyaniline and gold nanoparticles. Theelectrochemical characteristics of the electrodes were investigated by cyclic voltammetry, andelectroch...
متن کاملFabrication of sensitive enzymatic biosensor based on multi-layered reduced graphene oxide added PtAu nanoparticles-modified hybrid electrode
A highly sensitive amperometric glucose sensor was developed by immobilization of glucose oxidase (GOx) onto multi-layer reduced graphene oxide (MRGO) sheets decorated with platinum and gold flower-like nanoparticles (PtAuNPs) modified Au substrate electrode. The fabricated MRGO/PtAuNPs modified hybrid electrode demonstrated high electrocatalytic activities toward oxidation of H2O2, to which it...
متن کاملAn electrochemical biosensor for rapid detection of E. coli O157:H7 with highly efficient bi-functional glucose oxidase-polydopamine nanocomposites and Prussian blue modified screen-printed interdigitated electrodes.
The presence of pathogenic bacteria in foods has always been a great threat to the wellbeing of people and the revenue of food manufacturers. Therefore, the demand for advanced detection methods that can sensitively and rapidly detect these pathogens has been of great importance. This study reports an electrochemical biosensor for rapid detection of E. coli O157:H7 with the integration of bifun...
متن کاملOne-Pot Hydrothermal Synthesis of Magnetite Prussian Blue Nano-Composites and Their Application to Fabricate Glucose Biosensor
In this work, we presented a simple method to synthesize magnetite Prussian blue nano-composites (Fe₃O₄-PB) through one-pot hydrothermal process. Subsequently, the obtained nano-composites were used to fabricate a facile and effective glucose biosensor. The obtained nanoparticles were characterized using transmission electron microscopy, scanning electron microscopy, Fourier-transform infrared ...
متن کاملAmperometric biosensor based on Prussian Blue nanoparticle-modified screen-printed electrode for estimation of glucose-6-phosphate.
Glucose-6-phosphate (G6P) plays an important role in carbohydrate metabolism of all living organisms. Compared with the conventional analytical methods available for estimation of G6P, the biosensors having relative simplicity, specificity, low cost, and fast response time are a promising alternative. We have reported a G6P biosensor based on screen-printed electrode using Prussian Blue (PB) na...
متن کامل